Microcondensate-Mediated Intracellular Infusion of mRNA Across the Plasma Membrane.

ABSTRACT: This study presents a strategy for developing micrometer-sized coacervates comprised of cationic intracellular delivery peptide FcB(L17E)₃ and nucleic acids (termed "microcondensates") as a lipid nanoparticle (LNP)-free platform for intracellular messenger RNA (mRNA) delivery. The feasibility of this approach is demonstrated both in vitro and in vivo. This approach is based on our previous finding that FcB(L17E)₃ forms a microcondensate through interactions with IgG labeled with a negatively charged fluorescent dye. Similarly, the negative charge of nucleic acids causes siRNAs to form microcondensates with FcB(L17E)₃, resulting in the facile infusion of the siRNAs through the plasma membrane with 40% gene knockdown efficiency. mRNAs form aggregates with FcB

SUBMITTER: Kawaguchi Y 

PROVIDER: S-EPMC12759213 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature