IcmT is essential for pore formation-mediated egress of Legionella pneumophila from mammalian and protozoan cells.

ABSTRACT: The final step of the intracellular life cycle of Legionella pneumophila and other intracellular pathogens is their egress from the host cell after termination of intracellular replication. We have previously isolated five spontaneous mutants of L. pneumophila that replicate intracellularly similar to the wild-type strain but are defective in pore formation-mediated cytolysis and egress from mammalian and protozoan cells, and the mutants have been designated rib (release of intracellular bacteria). Here, we show that the rib mutants are not defective in the activity of enzymes secreted through the type II secretion system, including phospholipase A, lysophospholipase A, and monoacylglycerol lipase, although they are potential candidates for factors that lyse host cell membranes. In additio

SUBMITTER: Molmeret M 

PROVIDER: S-EPMC127627 | biostudies-literature | 2002 Jan

REPOSITORIES: biostudies-literature