Integrated transcriptome, metabolome and activity-guided fractionation of Parnassia wightiana Wall. reveal its insecticidal potential.

ABSTRACT:

Background

Parnassia wightiana Wall. (P. wightiana) is a traditional medicinal herb with demonstrated potential for developing eco-friendly botanical insecticides, but its insecticidal properties remain underexplored.

Methods

Here, we systematically evaluated the insecticidal activities of different plant tissues through integrated bioactivity assays and metabolomic profiling. Combining bioactivity-guided fractionation with transcriptomic analysis, we identified bioactive insecticidal compounds and elucidated key genes involved in secondary metabolite biosynthesis.

Results

Bioassays revealed that root extracts exhibited the highest insecticidal efficacy against third-instar Mythimna separata (M. separata) larvae, with a median lethal concentration (LC₅₀) of

SUBMITTER: Tang G 

PROVIDER: S-EPMC12763937 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature