Phytosphingosine contributes gut microbiota metabolism to alleviate low-grade endotoxemia-induced mastitis.

ABSTRACT:

Background

Recently, evidence has indicated that mastitis is closely associated with the ruminal dysbiosis caused by subacute ruminal acidosis (SARA) and subsequent low-grade endotoxemia (LGE). However, whether and how ruminal metabolic dysbiosis influences SARA-associated mastitis is still unclear.

Results

Using untargeted metabolomics, we found that cows with SARA-associated mastitis exhibited altered ruminal metabolic profiles, particularly a reduced level of phytosphingosine (PS), compared to healthy cows. Oral administration of PS to mice alleviated LGE-induced mastitis, as evidenced by attenuated mammary injury and improved function of mammary tight junctions (TJs). Furthermore, we demonstrated that LGE induced significant gut dysbiosis, characterized by increased abun

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC12764081 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature