Integrative genomic and machine learning approaches reveal evolutionary signatures in the winged bean mitochondrial genome.

ABSTRACT: The mitochondrial genome of Psophocarpus tetragonolobus (winged bean), a nutritionally valuable yet genomically underexplored tropical legume, was assembled using high-coverage PacBio long reads and Illumina short reads. The 366,925 bp circular genome encodes 64 genes (38 protein-coding, 20 tRNAs, 6 rRNAs) and contains nine fragmented protein-coding genes, indicative of dynamic mitogenome architecture. Repeat profiling revealed 100 dispersed repeats (30-110 bp) and 25 SSRs (4.95% of the genome), with assembly graph inspection and recombination models supporting subgenomic circles and isoforms. Comparative analyses across 15 legumes showed pervasive purifying selection, with positive selection in specific codons of atp4, ccmB, cox1, nad3, and rps10. Codon usage analyses showed that mitochon

SUBMITTER: Singh NK 

PROVIDER: S-EPMC12764132 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature