Integrative analysis of Helicobacter pylori-driven stomach adenocarcinoma reveals epigenetic deregulation, immune evasion, and therapeutic resistance.

ABSTRACT:

Background

Helicobacter pylori (H. pylori) infection is a major etiological factor for stomach adenocarcinoma (STAD), yet the key molecular drivers linking infection to tumor progression remain insufficiently defined. This study aimed to identify H. pylori-related hub genes in STAD and validate their functional relevance using integrated bioinformatics and experimental approaches.

Methodology

Differentially expressed genes (DEGs) were identified from two microarray datasets (GSE13911 and GSE54129) comparing H. pylori-positive STAD samples with controls. Common DEGs were used to construct a protein-protein interaction (PPI) network via STRING and Cytoscape, and hub genes were ranked using CytoHubba. Transcriptomic validation was conducted using TCGA-STAD data, followed by ana

SUBMITTER: Alissa M 

PROVIDER: S-EPMC12764169 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature