Whole genome of petroleum hydrocarbon degrading Rhodococcus indonesiensis isolated from Nacharam, Hyderabad, India.

ABSTRACT: Petroleum pollution poses a critical environmental concern. Bioremediation has gained prominence as an eco-friendly approach for mitigating hydrocarbon pollution. This study reports the isolation and comprehensive characterization of a novel petroleum-degrading bacterium, Rhodococcus indonesiensis SARSHI1. Whole-genome sequencing was performed using a hybrid approach, integrating Oxford Nanopore Technologies (PromethION) and Illumina (NovaSeq 6000) platforms. The complete genome spans 5.7 Mbp and an additional plasmid of 159,118 bp, together encoding 5,150 coding sequences. Structural annotation identified 5220 genes, including 5094 protein-coding genes, one non-coding RNA, one CRISPR array, 56 pseudogenes, and 243 hypothetical proteins. Sequencing yielded 13,900,477 Illumina and 2,539,063

SUBMITTER: Zaman SAU 

PROVIDER: S-EPMC12764523 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature