Thrombospondin-4 regulates apoptosis of vascular smooth muscle cells after artery transplanted into vein.

ABSTRACT: The molecular mechanism of vascular atrophy and remodeling after arterial transplantation into a venous environment remains unclear. This study grafted the common carotid artery of New Zealand white rabbits into the external jugular vein to establish an animal model of arterial mechanical unloading. Its efficacy was verified by ultrasound. Immunohistochemical methodologies, transmission electron microscopy, and TUNEL staining showed vascular morphology changes. Genomic analysis, proteomic profiling, iPathway guide (IPG) analysis, gene overexpression/silenced, flow cytometry, and stretch testing were applied to elucidate the molecular mechanisms. Comparative analysis between the arterial graft and the control group revealed the successful preparation of the animal model in vitro. Morphologi

SUBMITTER: Xu F 

PROVIDER: S-EPMC12764563 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature