A direct multiplex isothermal amplification-reverse dot blot hybridization system for β-thalassemia diagnosis.

ABSTRACT: Molecular diagnostic methods largely rely on expensive equipment and complex operations, which makes it difficult to achieve rapid and low-cost detection. In recent years, the increasing demand for point-of-care testing has driven the rapid development of isothermal amplification techniques, due to their simplicity and low equipment requirements. However, complex nucleic acid extraction steps are still required before most isothermal amplification. In this study, we propose a nucleic acid extraction-free gene detection method: direct multiplex Recombinase Aided Amplification combined with reverse dot blot hybridization (dmRAA-RDB). This method can detect multiple targets simultaneously and offers advantages such as high sensitivity, high specificity, low cost, no need for expensive instrum

SUBMITTER: Ye C 

PROVIDER: S-EPMC12764633 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature