High glucose enhances inflammation-driven platelet adhesion to endothelial cells in vitro.

ABSTRACT: Endothelial and platelet dysfunction are central to vascular disease development. We established a simplified, reproducible 96-well plate model to assess platelet adhesion to endothelial cells under conditions mimicking in vitro endothelial dysfunction and platelet hyperactivation. Human aortic endothelial cells (HAEC) were treated with tumour necrosis factor-alpha (TNF-α, 20-50 ng/mL) and/or high glucose levels (30 mM) to replicate in vitro the states of inflammation and hyperglycaemia. Treated HAEC were then exposed to platelets from healthy volunteers. In some experiments, platelets were treated with tirofiban, a GP IIb/IIIa inhibitor, before HAEC exposure. Platelet adhesion was evaluated by fluorescence, transmission, and scanning electron microscopy. Conditions showing significant eff

SUBMITTER: Scavone M 

PROVIDER: S-EPMC12764815 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature