Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells.

ABSTRACT: Broadly neutralizing monoclonal antibodies (bnAbs) against SARS-CoV-2 are crucial for developing therapeutics and vaccines that confer broad protection against SARS-CoV-2 variants and related sarbecoviruses. Here, we present a protocol to isolate bnAbs against SARS-CoV-2 from human B cells. We describe steps for sorting single spike-specific B cells from human peripheral blood mononuclear cells, cloning monoclonal antibodies (mAbs) from single B cells, screening mAbs with broadly neutralizing activity, and measuring the neutralizing potency of bnAbs against a panel of pseudoviruses. For complete details on the use and execution of this protocol, please refer to Chen et al.¹.

SUBMITTER: Huang X 

PROVIDER: S-EPMC12765176 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature