Barcoded viral tracing identifies immunosuppressive astrocyte-glioma interactions.

ABSTRACT: Glioblastoma (GBM) is the most lethal primary brain malignancy¹. Immunosuppression in the GBM tumour microenvironment (TME) is an important barrier to immune-targeted therapies, but our understanding of the mechanisms of immune regulation in the GBM TME is limited². Here we describe a viral barcode interaction-tracing approach³ to analyse TME cell-cell communication in GBM clinical samples and preclinical models at single-cell resolution. We combine it with single-cell and bulk RNA-sequencing analyses, human organotypic GBM cultures, in vivo cell-specific CRISPR-Cas9-driven genetic perturbations as well as human and mouse experimental systems to identify an annexin A1-formyl peptide receptor 1 (ANXA1-FPR1) bidirectional astrocyte-GBM communication pathway t

SUBMITTER: Andersen BM 

PROVIDER: S-EPMC12765215 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature