Investigation of the Difference in Growth-Promoting Factor Secretion Between Canine and Rabbit Corneal Epithelial Cells.

ABSTRACT:

Objective

We hypothesized that canine corneal epithelial cells maintain their proliferative properties through autocrine secretion of growth-promoting factors. This study aimed to investigate the maintenance mechanism of the proliferative properties of canine corneal epithelial cells by comparing the autocrine secretion of soluble factors in canine and rabbit corneal epithelial cells.

Procedures

The effect of conditioned medium from primary canine corneal epithelial cells and canine corneal epithelial cell line (cCEpi) on the growth of rabbit corneal epithelial cells was evaluated, and vice versa. cDNA microarray and quantitative polymerase chain reaction analyses were performed, and the gene expression of soluble factors in canine and rabbit corneal epithelial cells was com

SUBMITTER: Morita M 

PROVIDER: S-EPMC12765224 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature