Customized Surface Design to Increase Throughput of Ambient Ionization Mass Spectrometry Lipidomics.

ABSTRACT: Increased access to cheap and rapid mass spectrometry testing of biofluids is desirable for the analysis of disorders and diseases that may be linked to alterations in metabolite or lipid levels. The objective of this study is to establish an easily customized high-throughput workflow for the analysis of biological samples using desorption electrospray ionization-mass spectrometry (DESI-MS). The guiding principles of this workflow are the use of low-cost, open-source, and readily accessible materials with high-throughput and reproducibility. The design consists of 3 steps: (1) PARAFILM surface customization of size, shape, and depth of features on PARAFILM via 3D printed molds; (2) sample spotting via high-throughput robotics using the relatively inexpensive and open-source Opentrons platf

SUBMITTER: Goudarzi CC 

PROVIDER: S-EPMC12765230 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature