Viability of Dental Pulp Derived Stem Cells After Long-Term Cryopreservation.

ABSTRACT:

Introduction

Dental pulp-derived stem cells (DPSCs) have promise for use in regenerative therapies due to their regenerative and immunomodulatory potential. Following their isolation from dental pulp tissue, DPSCs can be cryopreserved and banked for future use, however, it remains unclear to what extent long-term storage affects their properties. This study evaluated the properties of DPSCs cryopreserved for up to 13 years.

Methods

DPSCs from 12 patients cryopreserved for 5 (DPSC-5 YR), 10 (DPSC-10 YR), and 13 (DPSC-13 YR) years were analyzed for viability, immunophenotype (CD34, CD45, CD73, CD90, CD105), proliferation, and stemness. Additionally, senescence was evaluated through gene expression and senescence-associated β-galactosidase activity.

Results

All cryoprese

SUBMITTER: Malik MA 

PROVIDER: S-EPMC12765376 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature