TET3 protects the <i>Dlk1-Dio3</i> imprinted locus from DNA hypomethylation during adult NSC reprogramming.

ABSTRACT: Genomic imprinting is an epigenetic mechanism that controls monoallelic expression according to parental origin. Imprinted genes are regulated by DNA methylation at imprinting control regions (ICRs), differentially methylated regions (DMRs) that distinguish between parental alleles. Cell reprogramming into induced pluripotent stem cells (iPSCs) offers a valuable model for studying pluripotency. Thus, discerning whether genomic imprinting changes during reprogramming represent epigenetic abnormalities or essential adaptations of pluripotency is crucial. Here, we integrate RNA-seq and MeDIP-seq analysis to profile mouse iPSCs derived from adult neural stem cells (NSCs). Our findings reveal that most ICRs undergo DNA hypomethylation in iPSCs, although the IG-DMR within the Dlk1-Dio3 im

SUBMITTER: Lazaro-Carot L 

PROVIDER: S-EPMC12765442 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature