Unraveling the Causal Linkages of <i>RBP7</i> and <i>SCGB3A1</i> on Pelvic Organ Prolapse: Multifaceted Insights From Genome-Wide Mendelian Randomization, Single-Cell RNA Analysis, and Network Pharmacology.

ABSTRACT:

Background

Pelvic organ prolapse (POP) is a common pelvic floor disorder in middle-aged and elderly women. Its pathophysiology is complex, involving weakened pelvic floor muscles and connective tissues. There is a need to explore its underlying pathogenesis and develop effective treatments.

Methods

We integrated single-cell sequencing (scRNA-seq) data analysis with Mendelian randomization (MR) analysis. scRNA-seq data of vaginal mucosal tissue were obtained from individuals with and without pelvic organ prolapse (POP); we then performed dimensionality reduction and cell subset identification. MR was conducted using GWAS summary statistics and eQTL data, following STROBE-MR guidelines. We also performed protein-protein interaction analysis, functional enrichment analysis, dru

SUBMITTER: Yang Y 

PROVIDER: S-EPMC12765987 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature