Spatiotemporal translation of sperm acrosome associated proteins during early capacitation modulates sperm fertilizing ability.

ABSTRACT:

Introduction

Despite the lack of essential cytoplasmic organelles in mature spermatozoa, which creates unfavorable conditions for transcription and translation, the presence of various mRNA and proteins during capacitation suggests potential for de novo protein synthesis.

Objectives

We applied a metabolic labeling method using a fluorescent noncanonical amino acid tagging system (FUNCAT) and proximity ligation method (PLA) in normal and reduced fertility spermatozoa to detect different translation phenomena during sperm capacitation according to their fertility.

Methods

We explored different proteome changes in spermatozoa according to the time-sequential capacitation process (0, 20, 40, 60, and 120 min) between normal [average fertility rate (FR) = 77.44 % ± 1.51] an

SUBMITTER: Park YJ 

PROVIDER: S-EPMC12766180 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature