Comparative single-cell transcriptomic map reveals divergence in leaves between two cotton species at cell type resolution.

ABSTRACT:

Introduction

Leaves are important functional organs in plants that determine yield and quality of crops. Upland cotton and sea-island cotton contribute more than 90% of the cotton fiber production annually. Deciphering and utilizing the diversity of leaf cells and functional genes underlying their divergences will be highly meaningful for cotton breeding.

Objectives

To investigate the conserved and divergent cell types of leaves between upland cotton and sea-island cotton, identify functional genes, and explore functional cell types in response to biotic and abiotic stresses in both species.

Methods

Nuclei were isolated from leaves of upland cotton CRI12 and sea-island cotton XH21, respectively, and single-nucleus RNA-seq (snRNA-seq) was performed. Based on the orthol

SUBMITTER: Cheng H 

PROVIDER: S-EPMC12766182 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature