Development of a fully human glioblastoma-in-brain-spheroid model for accelerated translational research.

ABSTRACT:

Introduction

Glioblastoma (GBM) progression and therapeutic resistance are significantly influenced by complex interactions between tumor cells and the brain microenvironment, particularly neurons. However, studying these interactions in physiologically relevant conditions has remained challenging due to limitations in existing model systems.

Objectives

Here, we present hGliCS (human glioma-cortical spheroid), a novel fully human brain tumor model that overcomes key limitations of current approaches by combining patient-derived GBM cells with mature human cortical neurons derived from induced pluripotent stem cells.

Results

We demonstrate that GBM cells in hGliCS develop three critical hallmark features observed in patients: (i) formation of tumor microtubes enabling

SUBMITTER: Horschitz S 

PROVIDER: S-EPMC12766203 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature