A mouse model of sepsis-associated DIC induced by Kappa-carrageenan and Lipopolysaccharides: Establishment and characteristics.

ABSTRACT:

Background

No animal models fully replicate the pathogenesis and clinical features of sepsis-associated disseminated intravascular coagulation (DIC), which hinders mechanistic understanding and treatment development. Kappa-carrageenan (KCG) and lipopolysaccharides (LPS) induce thrombosis and systemic inflammation in mice, respectively. The combination of LPS and KCG provides a promising method for establishing a mouse model of sepsis-associated DIC.

Objective

This study aimed to establish a standardized mouse model of sepsis-associated DIC using KCG and LPS.

Methods

Kunming (KM) mice were intraperitoneally injected with KCG (25-200 mg/kg) alone or in combination with LPS (50-1250 μg/kg) to determine optimal dose. The effects of ambient temperature, gender and mouse st

SUBMITTER: Tang P 

PROVIDER: S-EPMC12766204 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature