Blockade of calcium-activated chloride channel ANO1 ameliorates ionizing radiation-induced intestinal injury.

ABSTRACT:

Introduction

Radiation enteritis is one of the most frequent clinical complications of radiotherapy (RT), yet few effective strategies currently exist to protect against that. Anoctamin 1 (ANO1) functions both as a chloride channel and a signal transduction protein, influencing numerous pathophysiological processes.

Objectives

This study aimed to investigate whether targeting ANO1 could mitigate radiation-induced enteritis while enhancing tumor radiosensitivity.

Methods

Quantitative PCR (qPCR) and Western blot (WB) were used to assess ANO1 expression and its changes after irradiation. Survival rates were recorded to evaluate the effects of ANO1 agonist and inhibitors. A cystic fibrosis transmembrane conductance regulator (CFTR) inhibitor was administered to irradiated

SUBMITTER: Guo Y 

PROVIDER: S-EPMC12766212 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature