A Novel In-Cell ELISA With Superior Sensitivity and Specificity for the Detection of African Swine Fever Virus-Specific IgM and IgG Antibodies.

ABSTRACT: African swine fever (ASF), a high-profile transboundary animal disease caused by ASF virus (ASFV), imposes a devastating impact on the global swine industry. Given that vaccines are still under development, including field evaluations, early detection of ASFV is crucial for effective disease control and mitigation. Although PCR is the primary viral detection method of acute or subacute ASFV infections, antibody detection plays a unique role in detecting low-virulent ASFV infection, identifying recovered animals, and tracking viral transmission. ELISA for ASFV antibody detection is commonly used for initial serological screening. To avoid false positive results, the World Organisation for Animal Health (WOAH) recommends using a second serologic method, such as the indirect immunofluorescenc

SUBMITTER: Wu P 

PROVIDER: S-EPMC12766277 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature