Single-cell RNA profiling identifies immune cell population shifts in diabetes associated mucosal inflammation.

ABSTRACT: Poorly controlled diabetes significantly worsens periodontal disease, affecting millions worldwide, yet the mechanisms driving this destructive synergy remain unclear. We generated single-cell RNA sequencing profiles of diabetic periodontal tissue, revealing increased γ^δ T-cells, a loss of Tregs and greater neutrophil polarization as key mediators of diabetes-enhanced periodontitis. Flow cytometry confirmed significant expansion of IL-17A⁺ γ^δ+ T-cells and reduced Tregs in diabetic mice, with parallel findings of elevated CD3⁺IL-17A⁺ cells and reduced Tregs in human diabetic periodontal specimens. scRNAseq determined that diabetes caused a global increase in pro-inflammatory and a decrease in pro-resolving transcripts and enhanced infla

SUBMITTER: Alghamdi B 

PROVIDER: S-EPMC12766642 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature