A Structural Landscape Depiction of Dynamic Stability Centers of Local Structure in Protein Thermostability Engineering.

ABSTRACT: Engineering protein thermostability is a key aspect of rational protein design, aiming to broaden the applicability of enzymes and enhance their industrial utility. In this study, we introduce a strategy for identifying and reinforcing dynamic stability centers of local structure (DSCLSs) to improve protein thermostability. A DSCLS comprises key structural residues and their interactions, representing the structural basis of protein stability. Molecular dynamics, cross-correlation amino acid networks, and other analytical techniques were integrated into the method. This approach was initially inspired by thermostability engineering of exodiol dioxygenases (EDOs). The method was validated through mutational analyses of mesophilic EDO MT-2, CpKR (ketoreductase from Candida parapsilosis

SUBMITTER: Qiu X 

PROVIDER: S-EPMC12766707 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature