Single-cell structural lipidomics using a miniature dual-LIT mass spectrometer.

ABSTRACT: Structural lipidomics provides comprehensive information on lipidomes, and there is great interest in applying it to single-cell analysis for accurate cell phenotyping and lipid pathway studies. However, structural lipidomics relies on tandem mass spectrometry analysis of a large number of lipid species, which remains challenging for single-cell analysis due to the small sample amounts. Herein, using a miniature dual-linear ion trap (LIT) mass spectrometer with enhanced ion processing capability, we developed an effective strategy to achieve annotations of more than 100 lipid species in a single cell with high structural specificity. The ion utilization efficiency was greatly improved with multi-stage MS ⁿ (n = 2-4) analysis performed for each lipid species to acqu

SUBMITTER: Cai Z 

PROVIDER: S-EPMC12766753 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature