Caffeine Alleviates Oxidative Damage of Retinal Pigment Epithelium Cells.

ABSTRACT:

Purpose

The purpose of this study was to explore the protective effect of caffeine on oxidative damage of RPE cells and NaIO₃-induced retinal degeneration.

Methods

H₂O₂ was used to induce the oxidative damage in ARPE-19 cells. Cell viability was measured by the CCK-8 assay. The morphology of ARPE-19 cells was observed by optical microscope. The apoptosis of ARPE-19 cells was analyzed by Annexin V/PI staining, and DNA fragmentation was detected using the TUNEL assay. The protein levels of apoptosis markers BAX and BCL2 as well as senescence marker p21 were detected by Western blot. DNA damage was indicated by immunofluorescence staining of γ-H2AX and observed by fluorescence microscopy. Transcriptome profiling of ARPE-19 cells was performed by

SUBMITTER: Liu H 

PROVIDER: S-EPMC12767078 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature