Integrated protein and metabolic engineering enable high-yield biosynthesis of resveratrol derivatives in <i>Yarrowia lipolytica</i>.

ABSTRACT: Piceatannol and pterostilbene, two resveratrol derivatives with superior pharmacological properties, hold significant promise for therapeutic and nutraceutical applications. Despite significant progress in their biosynthesis from resveratrol, microbial de novo biosynthesis of these compounds remains underexplored. Here, we engineered Yarrowia lipolytica to achieve de novo production of piceatannol and pterostilbene from glucose via integrated protein and metabolic engineering. First, a high-yielding resveratrol-producing Y. lipolytica strain was constructed by optimizing heterologous pathway and overexpressing key enzyme, achieving 526.0 mg/L resveratrol. Then, the introduction of HpaBC monooxygenase from Pseudomonas aeruginosa produced 273.1 mg/L

SUBMITTER: Wu Y 

PROVIDER: S-EPMC12767179 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature