PU.1 inhibits the erythroid program by binding to GATA-1 on DNA and creating a repressive chromatin structure.

ABSTRACT: Transcriptional repression mechanisms are important during differentiation of multipotential hematopoietic progenitors, where they are thought to regulate lineage commitment and to extinguish alternative differentiation programs. PU.1 and GATA-1 are two critical hematopoietic transcription factors that physically interact and mutually antagonize each other's transcriptional activity and ability to promote myeloid and erythroid differentiation, respectively. We find that PU.1 inhibits the erythroid program by binding to GATA-1 on its target genes and organizing a complex of proteins that creates a repressive chromatin structure containing lysine-9 methylated H3 histones and heterochromatin protein 1. Although these features are thought to be stable aspects of repressed chromatin, we find th

SUBMITTER: Stopka T 

PROVIDER: S-EPMC1276718 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature