Profiling miRNAs involved in Human Oligodendrocyte Precursor Cell Differentiation and Maturation.

ABSTRACT: MicroRNAs (miRNAs) are evolutionarily conserved post-transcriptional regulators that play critical roles in cellular development and differentiation across species. Although the importance of miRNAs in oligodendrocyte lineage cell (OLLC) differentiation has been extensively studied in rodent models, their roles in human OL development remain less understood. To address this gap, we used a human embryonic stem cell (hESC) reporter system designed to study human OLs and OL progenitor cells (OPCs). Using an optimized differentiation protocol, we used the reporter hESCs to generate and isolate well-characterized OLLCs at specific developmental stages and performed next-generation sequencing-based miRNA profiling to identify stage-specific miRNAs enriched during OL lineage specification and mat

SUBMITTER: Barzegar M 

PROVIDER: S-EPMC12767315 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature