Stress-induced loss of CTCF reveals an alternative, promoter-based mode of cohesin looping.

ABSTRACT: Cells continually encounter environmental stressors that challenge homeostasis. How three-dimensional (3D) chromatin structure contributes to these stress responses, particularly under hyperosmotic conditions, remains poorly understood. Here, using time-resolved Hi-C, CUT&Tag, auxin-inducible depletion, and RNA-seq, we map 3D chromatin structure, its molecular drivers, and transcriptional outcomes during the hyperosmotic stress response. Within 1 hour of sorbitol treatment, pre-existing loops and domains undergo genome-wide collapse, accompanied by the emergence of several hundred de novo, sorbitol-induced loops that are more punctate, longer-range, and transient. These newly formed loops weaken over time and largely dissipate by 24 hours, coincident with recovery of pre-existing chromatin

SUBMITTER: Flores JP 

PROVIDER: S-EPMC12767365 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature