High-resolution binding data of TFIID and cofactors show promoter-specific differences <i>in vivo</i>.

ABSTRACT: TFIID is instrumental in recognizing promoter sequences and initiating transcription, yet a cohesive understanding of how this complex interacts with and functions at different promoter types in vivo is still lacking. Here, we employed ChIP-nexus to capture high-resolution binding footprints of all Drosophila TFIID subunits across the genome. These footprints reveal TFIID sub-modules whose DNA contacts suggest new structural details. At different promoter types, the footprints of the TAFs are very similar, suggesting the presence of engaged TFIID across all promoters. In contrast, the binding profile of TBP is promoter-specific, enabling us to identify TATA, DPR, and TCT/housekeeping promoters de novo, along with their underlying core promoter elements. Notably, our da

SUBMITTER: Alcantara SG 

PROVIDER: S-EPMC12767524 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature