Identification of senescence-associated drivers of tumour growth and progression using a novel microarray platform.

ABSTRACT: Senescence and the senescence associated secretory phenotype (SASP) are implicated in promoting early tumorigenesis but due to the complexity of SASP it has been difficult to identify the responsible factors. We used canonical SASP factors on our microenvironment microarray (MEMA) platform to systematically identify SASP-associated drivers of tumorigenesis in breast and lung cancer cells. We found multiple SASP factors enhanced the proliferation and overall cell numbers for both lung and breast cells grown on the MEMA, and that there was significant overlap in SASP-associated growth-promoting factors between the two different cell types. We validated the ability of several factors, including IL-6, TGF-β and EGF, to drive growth in in vitro assays. Interestingly, these factors were e

SUBMITTER: Ou HL 

PROVIDER: S-EPMC12767529 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature