Electrical pulse stimulation reflecting the episodic nature of real-life exercise modulates metabolic and secretory profile of primary human myotubes.

ABSTRACT: Electrical pulse stimulation (EPS) represents a useful tool to study exercise-related adaptations of muscle cells in vitro. Here, we examine the metabolic and secretory response of primary human muscle cells from metabolically healthy individuals to the EPS protocol reflecting the episodic nature of real-life exercise training. This intermittent EPS protocol alternates high-frequency stimulation periods with low-frequency resting periods. Continuous EPS was used as a comparator. Radiometric assessment of glucose and fatty acid metabolism was complemented by examination of mitochondrial OxPHOS proteins, fiber-type markers, and the release of selected myokines and extracellular vesicles into the media. Both EPS protocols facilitated glycogen synthesis and incomplete fatty acid oxidation (int

SUBMITTER: Gabrisova K 

PROVIDER: S-EPMC12767763 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature