Expression of major surface protein 2 variants with conserved T-cell epitopes in Anaplasma centrale vaccinates.

ABSTRACT: Major surface protein 2 (MSP-2), identified as a protection-inducing immunogen against Anaplasma marginale challenge, is an immunodominant outer membrane protein with orthologues in all examined Anaplasma species. Although immunization with live Anaplasma centrale has long been used to induce protection against acute disease upon challenge with virulent A. marginale, its MSP-2 structure and whether MSP-2 variants are generated during persistence of the vaccine strain was unknown. In this study, we showed that the A. centrale vaccine strain persisted for a minimum of 4 years postvaccination and generated sequential MSP-2 variants. Comparison of amino acid sequences encoded by A. centrale msp-2 transcripts from the initial postimmunization period and from sequential time points during persis

SUBMITTER: Shkap V 

PROVIDER: S-EPMC127678 | biostudies-literature | 2002 Feb

REPOSITORIES: biostudies-literature