All-in-one generation and multiomic profiling of human whole brain organoid on a millifluidic plate.

ABSTRACT: Human brain organoids (hBOs) have been recently regarded as neurobiologically relevant brain models and exponentially exploited in a variety of neuroscience research. However, the current gold-standard method for generating hBOs is intricate and laborious, resulting in hBOs with morphological variability and inconsistent batch-to-batch reproducibility. Despite several studies reporting simplified hBO culture methods, few of those methods was biologically validated with multiomic profiling, which is crucial for neurobiological studies. Here, we demonstrate an all-in-one millifluidic plate (AIOMP) with individually perfusable microchambers for hBOs, simplifying the culture process, improving the uniformity and reproducibility, and enabling long-term cultivation and real-time morphogenesis ob

SUBMITTER: Zhao W 

PROVIDER: S-EPMC12767812 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature