Unveiling Alternative Splicing in HPV-Negative HNSCC: Links to Tumorigenesis and Immune Landscape.

ABSTRACT:

Background

Alternative splicing (AS) plays crucial roles in tumorigenesis by regulating cancer development, progression, and patient outcomes. However, the prognostic value of AS in HPV-negative head and neck squamous cell carcinoma (HNSCC), a subtype with distinct clinical features and poor prognosis compared to HPV-positive HNSCC, remains underexplored. This study aimed to develop a robust predictive signature based on AS events in HPV-negative HNSCC.

Methods

We retrieved RNA-sequencing (RNA-seq) data and clinical profiles of the HNSCC cohort from The Cancer Genome Atlas (TCGA) via its data portal. Percentage Spliced In (PSI) values were derived from TCGA SpliceSeq. To identify prognostic AS events in HPV-negative HNSCC, we first performed univariate Cox analysis. Subseque

SUBMITTER: Li S 

PROVIDER: S-EPMC12767817 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature