Dual-Copy VP2 expressed with CTA1-DD in transgenic Eimeria acervulina confers partial protection against infectious bursal disease virus.

ABSTRACT: Infectious bursal disease (IBD), caused by the infectious bursal disease virus (IBDV), remains a major threat to global poultry production. The viral capsid protein VP2 is the primary protective antigen; however, conventional vaccines frequently provide only limited efficacy. In this study, we generated recombinant Eimeria acervulina strains expressing VP2 fused to the molecular adjuvant CTA1-DD (Cholera Toxin A1-D-domain dimer). Two transgenic strains were established: Ea-CVD (single-copy VP2) and Ea-VCVD (dual-copy VP2). Stable expression of the fusion protein was confirmed across multiple developmental stages, and both strains exhibited reduced fecundity relative to the wild-type parasite. Immunization trials in chickens showed that Ea-VCVD induced significantly stronger VP2-specific an

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12767827 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature