MASQOT: a method for cDNA microarray spot quality control.

ABSTRACT:

Background

cDNA microarray technology has emerged as a major player in the parallel detection of biomolecules, but still suffers from fundamental technical problems. Identifying and removing unreliable data is crucial to prevent the risk of receiving illusive analysis results. Visual assessment of spot quality is still a common procedure, despite the time-consuming work of manually inspecting spots in the range of hundreds of thousands or more.

Results

A novel methodology for cDNA microarray spot quality control is outlined. Multivariate discriminant analysis was used to assess spot quality based on existing and novel descriptors. The presented methodology displays high reproducibility and was found superior in identifying unreliable data compared to other evaluated methodol

SUBMITTER: Bylesjo M 

PROVIDER: S-EPMC1276784 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature