Understanding Enzalutamide-Resistance Based on a Functional Single-Cell Approach.

ABSTRACT:

Background

Anti-androgen or castration therapies are the mainstay treatment for metastatic prostate cancers (PCa). Although effective at first, androgen-dependent PCa (ADPC) universally develops therapy resistance, thereby evolving into an incurable disease called castration-resistant PCa (CRPC). Currently, mechanisms underlying the emergence of CRPC from ADPC are largely unclear.

Methods

We used single-cell RNA-sequencing (scRNA-Seq) to determine the transcription heterogeneity of a therapy-naïve ADPC cell line-LNCaP and how it responded to the anti-androgen drug, enzalutamide. Based on the results, we used single-cell/colony-based cloning to isolate a pre-enzalutamide cell subset, displaying low and/or no expression of androgen receptor (AR^low/-).

Results

SUBMITTER: Xue C 

PROVIDER: S-EPMC12768086 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature