Electric field-induced rupture kinetics of giant unilamellar vesicles with varying gramicidin A content in membrane.

ABSTRACT: In this study, we examine the rupture kinetics of giant unilamellar vesicles (GUVs) composed of DOPG/DOPC and varying mole fractions of gramicidin A (GrA), under externally applied electric fields. Time-resolved fluorescence microscopy reveals that the presence of GrA modulates vesicle rupture behavior in a non-monotonic fashion. At lower contents of GrA (0-0.05 mole%), GUVs exhibit enhanced structural resilience, with significantly reduced rupture probability, suggesting increased membrane stability. However, at higher contents of GrA (0.05-5%), vesicles display accelerated rupture kinetics, reflecting decreased membrane integrity. Quantitative analysis of rupture events indicates a biphasic dependence of the rupture rate constant on GrA content. The pore-edge tension was obtained from th

SUBMITTER: Bhuiyan MTI 

PROVIDER: S-EPMC12768252 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature