Improved birth rates via rehydration of mouse freeze-dried spermatozoa using high-temperature ultrapure water.

ABSTRACT: Freeze-drying (FD) is a promising method for achieving the long-term, low-cost, and safe preservation of mammalian sperm at room temperature (RT, 23-25°C). However, the birth rate of embryos fertilized with FD sperm is reduced to less than half compared to those fertilized with fresh sperm. Moreover, the underlying causes and potential solutions remain unclear. In this study, we investigated a rehydration process using FD sperm to determine its effects on sperm DNA damage. We also attempted to optimize this rehydration method to improve birth rates. We initially examined the effects of slowing water infiltration into mouse FD sperm using a high osmolarity or viscosity solution, but we found that this increased DNA damage and decreased birth rates. Next, to accelerate infiltration speed, we

SUBMITTER: Yamaji K 

PROVIDER: S-EPMC12768283 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature