ASM-chip: an antibody-modified size-screening microfluidic chip for high-efficiency circulating glioma cell isolation and clinical application.

ABSTRACT: Peripheral blood circulating glioma cells (CGCs) are crucial for glioma screening, prognostic evaluation, and diagnostic applications. However, reliable methods for efficient CGCs capture and comprehensive clinical evaluation on whether CGCs could faithfully represent complicated heavy equipment (such as MRI) remain insufficiently explored. Here, we present an antibody modification and size screening-based microfluidic (ASM) chip for effectively capturing CGCs, specifically designed for potential clinical translational applications. The ASM chip consists of one inlet, three outlets, one white blood cell clearance area, and one CGC capture area. The performance of the chip was optimized with two typical cell subtypes, namely SU-DHL-4 (to simulate leukocytes) and U251MG-ZsGreen (to simulate

SUBMITTER: Su H 

PROVIDER: S-EPMC12769480 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature