Weaning induces NOS-2 expression through NF-kappaB modulation in the lactating mammary gland: importance of GSH.

ABSTRACT: At the end of lactation the mammary gland undergoes involution, a process characterized by apoptosis of secretory cells and tissue remodelling. To gain insight into this process, we analysed the gene expression profile by oligonucleotide microarrays during lactation and after forced weaning. Up-regulation of inflammatory mediators and acute-phase response genes during weaning was found. Expression of IkappaBalpha (inhibitory kappaBalpha), a protein known to modulate NF-kappaB (nuclear factor-kappaB) nuclear translocation, was significantly up-regulated. On the other hand, there was a time-dependent degradation of IkappaBalpha protein levels in response to weaning, suggesting a role for NF-kappaB. Furthermore, we have demonstrated, using chromatin immunoprecipitation assays, binding of NF-k

SUBMITTER: Zaragoza R 

PROVIDER: S-EPMC1276959 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature