P4HA1 facilitates SPP1+ tumor-associated macrophages by activating the hypoxia pathway in head and neck squamous cell carcinoma.

ABSTRACT: Tumor-associated macrophages (TAMs) with highly expressed secreted phosphoprotein 1 (SPP1) carry immunosuppressive property as a potential target for tumor metastasis. However, the mechanisms regulating SPP1 + TAMs in head and neck squamous cell carcinoma (HNSCC) remain poorly understood. This study employs a combination of single-cell and bulk RNA sequencing bioinformatics analysis to confirm the impact of TAMs with high levels of SPP1 on patient prognosis. Additionally, Key genes linked to SPP1 + macrophages were identified using weighted gene co-expression network analysis. A prognostic model was built using the Random Forest algorithm. Here we show, P4HA1 is strongly correlated with SPP1 + macrophages and holds significant value in predicting patient prognosis and diagnosis. In vitro e

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12770402 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature