Dynamic microdevice culture during bovine oocyte maturation decreases lipid accumulation and improve blastocyst cell numbers.

ABSTRACT: Assisted reproductive technology (ART) enhances livestock fertility and genetic quality while reducing disease transmission. In vitro embryo production (IVEP) is a crucial technique that requires optimization. Microfluidics has emerged as a promising platform for IVEP by offering a controlled environment with a continuous nutrient supply. This study introduces a novel non-toxic and reusable microdevice with reversible sealing designed for the in vitro maturation (IVM) of bovine cumulus oocyte complexes (COCs). The microdevice is constructed from PMMA and PDMS_LAM on a glass slide, coated with Pluronic 167 F.The use of the microdevice for bovine IVM resulted in similar maturation rates (polar body extrusion rates) compared to the control (4-well-plate) (p = 0.73). Oocytes matured

SUBMITTER: Vit FF 

PROVIDER: S-EPMC12770460 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature