A transcriptome-wise Atlas of human prostate as a function of postmortem interval time.

ABSTRACT: The prostate gland is among the last internal organs to deteriorate during human decomposition. However, the effect of postmortem interval (PMI) on the mRNA and lncRNA expression and splicing is yet to be investigated in detail. The current study aims to identify the functional role of postmortem gene induction and pathway activation in prostate tissues with respect to the PMI gradient. Cadaver samples that were used in this study were collected during forensic autopsies and preserved at -20 °C in the morgue at the University of Pavia (Pavia, Italy). After RNA extraction, total RNA sequencing was performed on Illumina's NovaSeq 6000 using paired-ended sequencing approach. StringTie was used to perform expression level for mRNAs and lncRNAs by calculating FPKM. Additionally, mRNAs and lncRN

SUBMITTER: Javan GT 

PROVIDER: S-EPMC12770470 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature