Insights into de-mixing and morphology modulation in coacervate-membrane interactions from integrating experiments and simulations.

ABSTRACT: Intrinsically disordered proteins can undergo liquid-liquid phase separation to form condensates or coacervates, which play numerous regulatory roles in the cell. Recently, it was recognized that membrane-adsorbed condensates are crucial for biomolecular localization, and in some cases, induce significant changes in membrane morphology. A detailed understanding of the underlying mechanisms remains incomplete. Here, we combine experiments and simulations to unravel structural and dynamic features of the coacervate/membrane interface across scales. We study poly-Lysine/poly-Aspartate (K₁₀/D₁₀) coacervates with different unilamellar liposomes. By combining confocal microscopy, hyperspectral imaging, fluorescence recovery after photobleaching, and two complementary coarse

SUBMITTER: Mondal S 

PROVIDER: S-EPMC12770480 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature