Sodium butyrate-induced autophagy in colorectal cancer unveils the Ca²⁺/CaMKKβ signaling pathway as a potential therapeutic target.

ABSTRACT: Colorectal cancer's autophagy process is facilitated by sodium butyrate (NaB), but the mechanism remains unclear. This study aimed to elucidate the effects and underlying mechanisms of NaB-induced autophagy in colorectal cancer cells. In colorectal cancer cells, NaB has been shown to upregulate the expression of autophagy-related proteins, including microtubule-associated protein 1 A/1B-light chain 3 (LC3), thereby promoting autophagosome formation. Furthermore, NaB induces the activation of Ca²⁺/calmodulin-dependent kinase kinase β (CaMKKβ), AMP-activated protein kinase α (AMPKα), and acetyl-CoA carboxylase (ACC). Moreover, inhibiting CaMKKβ with STO-609 or downregulating CaMKKβ expression using RNA interference significantly attenuated the autophagic effect induced by NaB in c

SUBMITTER: Luo S 

PROVIDER: S-EPMC12770509 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature