Evaluating the practical aspects and performance of commercial single-cell RNA sequencing technologies.

ABSTRACT: The rapid development of updated and new commercially available single-cell transcriptomics platforms provides users with a range of experimental options. Cost, sensitivity, throughput, flexibility, and ease of use all influence the selection of an optimal workflow. We performed a comprehensive comparison of single-cell transcriptomic approaches using multiple standardized PBMCs from different donors. We report on standard single-cell metrics, including cell recovery, sequencing efficiency, sensitivity, cell annotation, and differential gene expression for seven recently available kits that interrogate whole transcriptome mRNA, and two that incorporate TCR profiling. We also discuss workflow throughput, sample requirements, timing, cost, and labor as critical factors to consider. Apart fro

SUBMITTER: Elz AE 

PROVIDER: S-EPMC12770963 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature